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Samples were transferred onto 0.5 μm-thick silicon nitride (Si 3 N 4 ) window (Silson), excess buffer wicked away and then the slide was frozen in liquid nitrogen (N 2 )-chilled liquid propane using a KF-80 plunge freezer (Leica Microsystems)

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Same , a compound naturally present in the body, supports liver function and contributes to the general well-being of the organism