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As an auxiliary method to HPLC, it improves the reliability of analytical results

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Precipitated proteins were separated by centrifugation and the concentrations of γ-glutamylcysteine in supernatants were determined by HPLC with coulometric detection using a Bio-Sil ODS-5S, 5-μm, 4.0 × 250 mm, C18 column (Bio-Rad, Life Science Research Group, Hercules, CA) eluted with a mobile phase consisting of 50 mM NaH 2 PO 4 , 0.05 mM octane sulfonic acid, 1% (v/v) acetonitrile and 0.5% N , N dimethylformamide (v/v) (pH 2.52) at a flow rate of 1 ml/min

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