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Laboratory research involving 5-Amino-1MQ has focused on selective NNMT inhibition and its effect on diet-induced obesity in mouse models (Learn more), and the downstream metabolic consequences of changing nicotinamide methylation, alongside related work on other longevity and metabolic compounds such as [NAD+]

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It took several more years before 5-amino-1MQ itself was tested as a compound, first in an 11-day mouse study, and the studies since then have stayed within the same species and the same short-to-moderate treatment windows