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One is your hypothalamic-pituitary-adrenal (HPA) system that releases a series of hormones, ending with the secretion of cortisol from the adrenal glands

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Thermo Fisher Scientific) against one litre of TBS (50 mM Tris–HCl pH 7.4, 150 mM NaCl) with a magnetic stirrer at 4 °C for 16 h, followed by a second dialysis with a magnetic stirrer at 4 °C for 2 h