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Recombinant proteins were separated using Bolt 4–12% Bis-Tris or NuPAGE 3–8% Tris-Acetate gels and transferred to nitrocellulose membranes with the iBlot 2 Dry Blotting System (all Thermo Fisher Scientific)

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These preliminary reports suggest that short-term, moderate-dose ALA supplementation was tolerated in pregnancy and may have some beneficial metabolic effects (e.g., improved blood sugar control or liver enzyme levels in gestational diabetes)