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At this stage of purification GST-X fusion should be clean and depending on the nature of furhter experiments that we want to perform, we can either elute the fusion with glutathione (excess of the glutathione will compete and displace GST-X protein from the beads) or cleave the GST tag and release protein X (see cartoon below)

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Form:Suppositories

Effect of cigarette smoking on levels of seminal oxidative stress in infertile men: a prospective study