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Rather than elute the protein with glutathione buffer, a PBS solution containing the enzyme is loaded onto the column and incubated for several hours at room temperature (4 °C for PreScission protease)

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the combined cognitive and function score differed by 0.08 points (p 0.70), ADAS-Cog11 by 0.70 points (p 0.35) and the daily-living scale by 0.67 points (p 0.61)